Wendan He, Boyue Deng, Zhongdong Wang, Xiaoxia Che
Abstract
Peri-miniscrew implant inflammation (PMSII) is a common complication after orthodontic anchorage, causing chronic progressive inflammation. This study aimed to investigate the role of miR-125a-5p in the regulation of macrophage polarization. THP-1 cells were induced to differentiate into macrophages. We modulated miR-125a-5p levels in THP-1 cells through mimics and inhibitors and cocultured these with HGF-1 cells to simulate the physiological environment. Our results indicated that increased miR-125a-5p levels in THP-1 cells induced M2-like macrophage polarization, enhanced cell proliferation and stimulated cell migration, whereas these effects were counteracted by miR-125a-5p knockdown. Bioinformatics analysis predicted target genes of miR-125a-5p that were enriched in the HIF-1 pathway, and differential expression of miR-125a-5p regulated the activity of related proteins in this pathway. Crosstalk effect of miR-125a-5p-induced macrophage polarization on HGF-1 cells revealed that more cells lost their spindle shape and were closer to a round shape in miR-125a-5p high-expressing THP-1 cocultures with HGF-1. Further studies suggested that miR-125a-5p was involved in the growth and proliferation of gingival fibroblasts through macrophages. Collectively, our findings suggest that miR-125a-5p is a potential target for regulating macrophage polarization, which provides new insights for the clinical treatment of peri-implantitis.
DOI : 10.62610/RJOR.2025.1.17.30
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