Carmen (Amititeloaie) Gentimir, Gabriela Geleţu, Andreea (Tibeică) Iordan, Marcel Costuleanu
ABSTRACT
Aim of the study The fast development of the tumor mass in acute lymphoblastic leukemia is dependent not only on the rate of cell proliferation, but also on the rate of apoptosis, with NR4A1 governing both tumor cell survival and death. Material and methods Treatment with 10 µm Cytosporone B was utilized to induce pro-B cell apoptosis, with the latter being delivered in the culture medium for 48 hours. The active chemical reagents we used for our detailed experiments and protocols were as follows: 1 µm IAXO-102 (a TLR4 antagonist); 1 µm INH14 (TLR2 functional inhibitor); and 1 µm IL-7 (a promotor of pro-B transformation into pre-B cells). Results The real and distinguishable most important effects of increasing apoptosis of pro-B lymphocytes, induced by Cytosporone B when using microvesicles expelled by gingival fibroblasts, were linked to the following treatments, in reducing order: IAXO-102, IL-7, and INH14. Conclusions TLR4 and TLR2 inhibition, as well as IL-7 stimulation, may increase the apoptotic degree generated by Cytosporone B activation of NUR77/NR4A1 orphan receptors, as well as the presence of microvesicles released by gingival fibroblasts.
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